you know how it goes
yes yes...you all know how it goes...
after i stop writing on my blog for a bit, it goes through this very "drought" like stage, during which time i DO NOT WRITE...and say that i will.
i don't know. summer is pretty boring for me-- i wish i had more to say than that. work is going well. i clocked in about 75 hours over a couple weeks, which has been able to rake me in a good 700 bucks or so. too bad its not NEARLY enough money to feed my insatiable needs to spend.
so, here's what i'm doing in the lab: (disclaimer: what i am doing sounds a lot harder than it really is. in all actuality, what i do is quite simple...its like cooking, but on a microscopic level) so...here's a run down of what i am doing. there are 3 "players" in this situation, there's BAD, PP2A, and 1433. each one of the players are being tagged with two different molecules: ECFP and EYFP. these molecules, when close to one another (within 50 angstroms to be exact), will transfer energy from one to the other. this reaction is called "FRET", an acronym for "Fluorescent (or Forster) Resonance Energy Transmission". so, in order to tag these "players" with the fluorescent molecules, you have to manipulate the DNA of the players by taking a piece of the DNA out and adding in the fluorescent molecule that you want. with BAD, PP2A and 1433, two of them will be paired with each other at any time, and i am trying to figure out when they pair together, when they break off, and what causes them to break apart: e.g. are two of them fighting for one site?...does one just leave a site for the other to attach?...etc. once the players have the fluorescent molecule added on, the tagged DNA is injected into BOSC cells. BOSC cells take in the DNA, and package retrovirus-es that have the tagged DNA in them by using materials from the BOSC cells. finally, these newly created retroviruses are used to infect mammalian cells: the retroviruses will go infect the mammalian cell, add its DNA to the nucleus, and the cell with then create the fluorescent molecules that we initially made. then you used a FRET microscope to read and see the pretty fluorescent colors!!
cool, huh? all this work is being done in the pediatric oncology and hematology dept. so i feel all warm and fuzzy that i am doing cell apoptosis research (pretty much cancer research). saving the world, one cell at a time!
i've really enjoyed work and i've been learning tons! the people in the lab are really cool too.
yesterday, i experience an interesting situation that involved my mother taking my car from where i parked it...and not telling me...in this "Punk'd" episode of sorts...minus the fact that i needed the car to take a bunch of people to a birthday lunch that our boss was throwing...and me looking really stupid because i had 3 people riding with me...and we all ended up getting to the party an hour late...
needless to say, i was PISSED at my mom for not telling me she took the car; she, on the other end, thought it was hilaaaarious.
anyway, here's a big ol' fuck you to bapple and RB, who both came to my house, ditched me to butt fuck each other under the excuse "we're going to watch the chapelle show", and left me to lonely and bored by myself. i just went to bed as they enjoyed themselves in the living room.
my vow: never hanging out with either of those two again.
...which means my social networking has gone down ten fold, and i'll be hanging a lot out by myself.
waaaah-- i miss miss yale! i miss it as it seems like the social scene is evolving without me, and by the time i get back to school, i'll be out like those von dutch hats. :( i can only hope and pray that this will not happen.
a few things to be thankful for: thank GOD for the "ghost" function on aim.
o yea- my obsession for this week: BATTLE ROYALE. maybe the best japanese film ever made...its a twisted tale of 42 teenage high school students, sent to an island by the japanese government, to kill one another in a "survival of the fitteest" until only ONE is left on teh island. i don't know what it is about this impeccably written movie, but i am in love with it...
here's something cool: i'm ebaying a digital camera...an optio s4i to be exact; small enough to fit in an altoid can, which is REALLY important...right...? i also want to get myself an ipod mini, but that will take a bit longer since they are 250 fucking dollars!!! jeebus.
anyway, i'm rambled on enough.
that's my story, and i'm sticking to it.
after i stop writing on my blog for a bit, it goes through this very "drought" like stage, during which time i DO NOT WRITE...and say that i will.
i don't know. summer is pretty boring for me-- i wish i had more to say than that. work is going well. i clocked in about 75 hours over a couple weeks, which has been able to rake me in a good 700 bucks or so. too bad its not NEARLY enough money to feed my insatiable needs to spend.
so, here's what i'm doing in the lab: (disclaimer: what i am doing sounds a lot harder than it really is. in all actuality, what i do is quite simple...its like cooking, but on a microscopic level) so...here's a run down of what i am doing. there are 3 "players" in this situation, there's BAD, PP2A, and 1433. each one of the players are being tagged with two different molecules: ECFP and EYFP. these molecules, when close to one another (within 50 angstroms to be exact), will transfer energy from one to the other. this reaction is called "FRET", an acronym for "Fluorescent (or Forster) Resonance Energy Transmission". so, in order to tag these "players" with the fluorescent molecules, you have to manipulate the DNA of the players by taking a piece of the DNA out and adding in the fluorescent molecule that you want. with BAD, PP2A and 1433, two of them will be paired with each other at any time, and i am trying to figure out when they pair together, when they break off, and what causes them to break apart: e.g. are two of them fighting for one site?...does one just leave a site for the other to attach?...etc. once the players have the fluorescent molecule added on, the tagged DNA is injected into BOSC cells. BOSC cells take in the DNA, and package retrovirus-es that have the tagged DNA in them by using materials from the BOSC cells. finally, these newly created retroviruses are used to infect mammalian cells: the retroviruses will go infect the mammalian cell, add its DNA to the nucleus, and the cell with then create the fluorescent molecules that we initially made. then you used a FRET microscope to read and see the pretty fluorescent colors!!
cool, huh? all this work is being done in the pediatric oncology and hematology dept. so i feel all warm and fuzzy that i am doing cell apoptosis research (pretty much cancer research). saving the world, one cell at a time!
i've really enjoyed work and i've been learning tons! the people in the lab are really cool too.
yesterday, i experience an interesting situation that involved my mother taking my car from where i parked it...and not telling me...in this "Punk'd" episode of sorts...minus the fact that i needed the car to take a bunch of people to a birthday lunch that our boss was throwing...and me looking really stupid because i had 3 people riding with me...and we all ended up getting to the party an hour late...
needless to say, i was PISSED at my mom for not telling me she took the car; she, on the other end, thought it was hilaaaarious.
anyway, here's a big ol' fuck you to bapple and RB, who both came to my house, ditched me to butt fuck each other under the excuse "we're going to watch the chapelle show", and left me to lonely and bored by myself. i just went to bed as they enjoyed themselves in the living room.
my vow: never hanging out with either of those two again.
...which means my social networking has gone down ten fold, and i'll be hanging a lot out by myself.
waaaah-- i miss miss yale! i miss it as it seems like the social scene is evolving without me, and by the time i get back to school, i'll be out like those von dutch hats. :( i can only hope and pray that this will not happen.
a few things to be thankful for: thank GOD for the "ghost" function on aim.
o yea- my obsession for this week: BATTLE ROYALE. maybe the best japanese film ever made...its a twisted tale of 42 teenage high school students, sent to an island by the japanese government, to kill one another in a "survival of the fitteest" until only ONE is left on teh island. i don't know what it is about this impeccably written movie, but i am in love with it...
here's something cool: i'm ebaying a digital camera...an optio s4i to be exact; small enough to fit in an altoid can, which is REALLY important...right...? i also want to get myself an ipod mini, but that will take a bit longer since they are 250 fucking dollars!!! jeebus.
anyway, i'm rambled on enough.
that's my story, and i'm sticking to it.
posted by Joe @ 7:44 PM
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